WebApr 20, 2024 · The PCR product was incubated with EcoRI and NotI (Thermo Scientific, Waltham, Massachusetts, USA) at 37 °C for 0.5 h. After purification by GeneJET Gel Extraction Kit (Thermo Scientific, Waltham, Massachusetts, USA), the PCR product was ligated to EcoRI-and NotI-digested vector driven by EF1α promoter by T4 ligase. WebMay 24, 2024 · The ribonucleoprotein complex was created by adding the Cas9 nuclease, and this complex was transfected into 400 000 HEK293T cells per well in a 12-well plate using Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Thermo Scientific) in Opti-MEM Reduced Serum Medium (Thermo Scientific).
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WebMar 3, 2024 · Thermo Fisher Scientific: Cat#K0441: GeneArt Platinum Cas9 Nuclease: Invitrogen: Cat#B25640: Lipofectamine LTX: Invitrogen: Cat#15335-100: Puromycin: Sigma-Aldrich: ... Obtained fragments were cloned into BsptI and NotI sites (for Q1230A substitution) or EcoRI and NotI sites (for R1576A, N1580A and R1576A/N1580A … WebJan 31, 2024 · For the zebrafish hcar1-4 or human OXER1 rescue experiments, pCS2+hcar1-4-mKate2 or pCS2+OXER1-mKate2 were linearized using FastDigest NotI (Thermo Fisher Scientific; FD0593), and in vitro transcribed using the mMessage mMachine SP6 kit (Thermo Fisher Scientific; AM1340). 2.3 nL of ∼500 ng/μL mRNA was injected into the yolk of one … dhsc workforce fund
NotI (10 U/µL)
WebConvenient systems for the routine subcloning of PCR products $232.50 - $451.50 Specifications View More Specs Includes: Vector, Control insert DNA, T4 DNA ligase, Ligation buffer, High efficiency competent cells (Mfr. Nos. A3610 and A1380 only) Products 4 Description Specifications Description WebChina) between the XhoI and NotI (Thermo Fisher Scientific, Waltham, MA) sites to construct the psiCHECK-2-3′UTR-C and psiCHECK-2-3′UTR-T dual-luciferase re- ... (Thermo Fisher Scientific). Cell proliferation assay Cell Counting Kit-8 (CCK-8) assay was performed according to the protocol described by Xu WebThermo Scientific™ NotI (10 U/µL) The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI). Supplier: Thermo … cincinnati bengals zoom background